Map the complete landscape of histone modifications across the entire genome with unparalleled resolution.
HisTLAND®
Product: HisTLAND® Histone Modifications Kit v1.1.
Description: A complete, integrated system designed for genome-wide histone modifications profiling.
Efficiency: Streamlined less than 24h protocol with a total hands-on time of less than 8h.
Input: Optimized for 100,000-250,000 cells per sample.
A Streamlined Approach from Nuclei to Library
WORKFLOW
Genome-wide Coverage
Comprehensive maps of histone marks
Validated Protocol
QC-tested, reproducible results
Multimodal Compatible
Profile multiple marks per sample
Protocol overview
NGS-Ready
STEP 01
Library ready for sequencing
Nuclei extraction
STEP 02
Complex binding overnight
Capture and
Pre-Amplification
Library Preparation
STEP 03
STEP 04
STEP 05
NGS and Data Analysis
SCIENTIFIC PUBLICATIONS
Multimodal nanoCUT&Tag in human CNS - first single-cell histone modification atlas of the adult human CNS, revealing dormant developmental programmes that can be hijacked in brain cancer.
Kabbe, M., … Castelo-Branco, G. Single-nucleus epigenomic profiling of the adult human central nervous system unveils epigenetic memory of developmental programs, 29, 992–1006.
https://www.nature.com/articles/s41593-026-02208-0
First publication with single-cell multimodal nanoCUT&Tag in Drosophila - resolves epigenetic landscapes of Drosophila embryo genesis.
Pirogov, S., … Bartosovic, M.,Mannervik, M. Single-cell chromatin landscapes visualize epigenetic barriers and reveal lineage-specific Polycomb-mediated repression.
https://www.biorxiv.org/content/10.64898/2026.05.04.722686v1
2026
Multimodal nanoCUT&Tag (base for HisTLAND) - first publication describing the multimodal profiling of two histone modifications and open chromatin in the same cell at the single cell resolution.
Bartosovic, M. & Castelo-Branco, G. Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Nature Biotechnology, 41, 794–805.
https://www.nature.com/articles/s41587-022-01535-4
2023
Spatial CUT&Tag - first publication describing the spatial application.
Deng, Y., Bartosovic, M. et al.. Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level. Science, 375(6581), 681–686.
https://doi.org/10.1126/science.abg7216
Spatial CUT&Tag-RNAseq - first publication describing the combined analysis of histone modification and RNA seq at the spatial resolution.
Zhang, D.,… Bartosovic, M. et al.. Spatial epigenome–transcriptome co-profiling of mammalian tissues. Nature, 616, 113-132.
https://doi.org/10.1038/s41586-023-05795-1
2022
Single-cell CUT&Tag - first publication describing the single-cell technology.
Bartosovic, M., Kabbe, M. & Castelo-Branco, G. Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues. Nature Biotechnology, 39(7), 825–835.
https://doi.org/10.1038/s41587-021-00869-9